Fatty acids in phospholipids of cells, cysts, and germinating cysts of Azotobacter vinelandii.
نویسندگان
چکیده
Cyclopropane fatty acids constitute 25% of the phospholipid acyl groups in cysts of Azotobacter vinelandii. These are lost by dilution during germination when the synthesis of the fatty acids characteristic of vegetative cell phospholipids commences.
منابع مشابه
Protein turnover in Azotobacter vinelandii during encystment and germination.
Protein turnover occurs during differentiation of Azotobacter vinelandii 12837 to the extent of 50% during encystment and 7% during germination. The addition of rifampin at the initiation of encystment prevents encystment and inhibits turnover. In germinating cysts, protein turnover is essential owing to an apparent lack of certain amino acid biosynthetic enzymes. The capacity to synthesize sul...
متن کاملIsolation and characterization of Azotobacter vinelandii mutants impaired in alkylresorcinol synthesis: alkylresorcinols are not essential for cyst desiccation resistance.
During encystment of Azotobacter vinelandii, a family of alkylresorcinols (ARs) and alkylpyrones (APs) are synthesized. In the mature cyst, these lipids replace the membrane phospholipids and are also components of the layers covering the cyst. In this study, A. vinelandii strains unable to synthesize ARs were isolated after mini-Tn5 mutagenesis. Cloning and nucleotide sequencing of the affecte...
متن کاملGiant cysts and cysts with multiple central bodies in Azotobacter vinelandii.
Cyst germination in Azotobacter vinelandii ATCC 12837 was studied by using phase contrast and electron microscopy. Germination in this organism was accompanied by the formation of large cyst forms of two different types: giant cysts and cysts containing multiple central bodies. Previously, these two types have been reported only when yeast extract was added to the encystment medium. In this stu...
متن کاملFormation of multiple central bodies in giant cysts of Azotobacter vinelandii.
Cysts of Azotobacter vinelandii produced in chemical salts-agar supplemented with 0.1 to 1.0% yeast extract and 0.3% butanol (L. E. Jackson, M.S. Thesis, Univ. of Texas, Austin, 1961) were two to four times larger than cysts formed on nitrogen-free medium (Fig. 1 and 2). Microscopic studies of "giant" cysts transferred to nitrogenfree agar revealed that, prior to germination, the central body d...
متن کاملA small heat-shock protein (Hsp20) regulated by RpoS is essential for cyst desiccation resistance in Azotobacter vinelandii.
In Azotobacter vinelandii, a cyst-forming bacterium, the alternative sigma factor RpoS is essential to the formation of cysts resistant to desiccation and to synthesis of the cyst-specific lipids, alkylresorcinols. In this study, we carried out a proteome analysis of vegetative cells and cysts of A. vinelandii strain AEIV and its rpoS mutant derivative AErpoS. This analysis allowed us to identi...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 137 3 شماره
صفحات -
تاریخ انتشار 1979